Mohammad Pichand; Ghasem Ali Dianati Tilaki; Ehsan Sadati
Abstract
To evaluate the effects of hydropriming and drought stress on germination traits of cymbopogon olivieri the experimental design was two factors factorial arranged in a completely randomized design, with four replications and 50 seeds per replicate. The first factor was hydropriming (in four levels), ...
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To evaluate the effects of hydropriming and drought stress on germination traits of cymbopogon olivieri the experimental design was two factors factorial arranged in a completely randomized design, with four replications and 50 seeds per replicate. The first factor was hydropriming (in four levels), the second, polyethylene glycol 6000 (in four levels), in laboratory conditions. That data were analyzed using spss software. The difference between the means was compared using duncan test (p<0.05). ANOVA Results indicated that the interaction between hydropriming time treatment and polyethylene glycol treatment caused significant differences in germination rate, shoot length, shoot wet and dry weight (P<0.01) and vigor index (P<0.05 ). But it caused no significant differences in parameters of percentage of germination, average germination time, root length, root wet and dry weight. The highest percentage of germination was related to the treatment by -4 bar PEG and without priming (control). The lowest percentage of germination was resulted under hydropriming in 48h and treatment by -8 bar polyethylen glycol respectively 49 and 14 percent. The highest rate of germination was related to the treatment by -8 bar PEG and hydropriming in 48h. Also seeds didn’t germinated in -16 bar of PEG in all hydropriming times. The results showed that the treatments by -4 and -8 bar PEG during 48 and 72 hours hydropriming caused the most positive effects on seed germination percentage, germination rate, vigor index, root length, shoot length of cymbopogon olivieri seeds.
Ghasem Ali Dianati Tilaki; Aliyeh Keshavarz; Bahram Amiri; Ehsan Sadati
Abstract
Effect of salt stress on the stomata variations was studied in Hedysarumcoronarium L. and HedysarumcriniferumBoiss., using optical microscope. The experimental design was a two factorial (species treatments and salt concentrations including 0, 100, 200, 250 mMNaCl) which arranged in a completely randomized ...
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Effect of salt stress on the stomata variations was studied in Hedysarumcoronarium L. and HedysarumcriniferumBoiss., using optical microscope. The experimental design was a two factorial (species treatments and salt concentrations including 0, 100, 200, 250 mMNaCl) which arranged in a completely randomized design with four replicates and 50 seeds per replicate. Seeds were grown in plastic pots with sterile sand. All pots were irrigated by distilled water until germination stage. Then the pots were irrigated uniformly every other day with modified Hoagland’s nutrient solution during 40 days and finally stomata traits were measured. The data was analyzed using SPSS software. The difference between the means was compared using Duncan test (P<0.05). Results showed that the lowest amount of the stomata length, stomata width and area of stomata were observed at 300 Mm salinity stress. Length and width of stomata were reduced by increasing salinity stress. The length stomata decreased 22.33 and 23.76 (μm) for H. coronarium and H. criniferum respectively, while width stomata decreased by 17.13 and 18.62 (μm) respectively. The area of stomata decreased 397.11 and 442.83 (μm²) for H. coronarium and H. criniferum respectively. Salinity stress had positive effect on stomata density (mm²). The highest density of stomata was found in H. criniferumin 250 and 300 mM salinity levels.
