Effects of Salinity Stress on Stomatal Variations of Hedysarum coronarium L. and Hedysarum criniferum Boiss.

Dianati Tilaki, Ghasem Ali; Keshavarz, Aliyeh; Amiri, Bahram; Sadati, Ehsan

doi:10.22059/jrwm.2017.61969

Document Type : Research Paper

Authors

¹ Associate Professor of Tarbiat Modares University

² Tarbiat Modares University

³ Islamic Azad University, Firoozabad Branch, Iran

⁴ Mazandaran, Research Center of Agricultural and Natural Resources

https://doi.org/10.22059/jrwm.2017.61969

Abstract

Effect of salt stress on the stomata variations was studied in Hedysarumcoronarium L. and HedysarumcriniferumBoiss., using optical microscope. The experimental design was a two factorial (species treatments and salt concentrations including 0, 100, 200, 250 mMNaCl) which arranged in a completely randomized design with four replicates and 50 seeds per replicate. Seeds were grown in plastic pots with sterile sand. All pots were irrigated by distilled water until germination stage. Then the pots were irrigated uniformly every other day with modified Hoagland’s nutrient solution during 40 days and finally stomata traits were measured. The data was analyzed using SPSS software. The difference between the means was compared using Duncan test (P<0.05). Results showed that the lowest amount of the stomata length, stomata width and area of stomata were observed at 300 Mm salinity stress. Length and width of stomata were reduced by increasing salinity stress. The length stomata decreased 22.33 and 23.76 (μm) for H. coronarium and H. criniferum respectively, while width stomata decreased by 17.13 and 18.62 (μm) respectively. The area of stomata decreased 397.11 and 442.83 (μm²) for H. coronarium and H. criniferum respectively. Salinity stress had positive effect on stomata density (mm²). The highest density of stomata was found in H. criniferumin 250 and 300 mM salinity levels.

Keywords

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Journal of Range and Watershed Managment

Effects of Salinity Stress on Stomatal Variations of Hedysarum coronarium L. and Hedysarum criniferum Boiss.

References

References

Volume 70, Issue 1
June 2017
Pages 101-110

Effects of Salinity Stress on Stomatal Variations of Hedysarum coronarium L. and Hedysarum criniferum Boiss.

References

References

Volume 70, Issue 1June 2017Pages 101-110

Volume 70, Issue 1
June 2017
Pages 101-110