Document Type : Research Paper


1 Associate Professor of Tarbiat Modares University

2 Tarbiat Modares University

3 Islamic Azad University, Firoozabad Branch, Iran

4 Mazandaran, Research Center of Agricultural and Natural Resources


Effect of salt stress on the stomata variations was studied in Hedysarumcoronarium L. and HedysarumcriniferumBoiss., using optical microscope. The experimental design was a two factorial (species treatments and salt concentrations including 0, 100, 200, 250 mMNaCl) which arranged in a completely randomized design with four replicates and 50 seeds per replicate. Seeds were grown in plastic pots with sterile sand. All pots were irrigated by distilled water until germination stage. Then the pots were irrigated uniformly every other day with modified Hoagland’s nutrient solution during 40 days and finally stomata traits were measured. The data was analyzed using SPSS software. The difference between the means was compared using Duncan test (P<0.05). Results showed that the lowest amount of the stomata length, stomata width and area of stomata were observed at 300 Mm salinity stress. Length and width of stomata were reduced by increasing salinity stress. The length stomata decreased 22.33 and 23.76 (μm) for H. coronarium and H. criniferum respectively, while width stomata decreased by 17.13 and 18.62 (μm) respectively. The area of stomata decreased 397.11 and 442.83 (μm²) for H. coronarium and H. criniferum respectively. Salinity stress had positive effect on stomata density (mm²). The highest density of stomata was found in H. criniferumin 250 and 300 mM salinity levels.


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