Although a number of studies have been carried out around meteorological drought in Isfahan province, there is no specific study about hydrologic drought. In this study, low flows trend was analysed for the first time as the most important indicator of hydrologic drought in Isfahan province watersheds. Mean daily stream flow as a hydrologic indicator and 1-, 7-, 15-, 30- and 90-day low flows were estimated as hydrologic drought indicators for 22 gauging stations with at least 20 years of data record. Results of trend analysis using Mann-Kendall and Spearman correlation indicated that mean daily stream flow and low flows have negative trend at many gauging stations. Apart from GhalehShahrokh station, low flow indices in Zayandehrud dam basin have negative trend so that Eskandari station shows the highest degree of negative trend. Daily stream flow and low flows reduction especially in recent years is kind of warning for water resources management in Zayandehrud basin.