The symbiosis of arbuscular mycorrhizal fungi with plants root plays an important role in the sustainability and development of medicinal plant communities in ecosystems which are subject to various physical and ecological pressures.Teucrium polium L. is one of the most important medicinal plants that exists in these areas. In this study after identifying three species of mycorrhizal fungi consist of G. rosea، S. constrictum، G. intraradices which were abundant in the soil of the root zone, The spores were propagated separately and a combination of fungi. Then spores were inoculated with T. polium seeds in a completely randomized design with three replications under greenhouse conditions. After the end of growth period, results showed that the highest stem length was related to G. intraradices. The highest fresh and dry weight of shoots (1.61 and 4.34 g) and root dry weight (0.7 g) were related to combined fungi treatment that showed a significant difference with other treatments. Also the combination of fungi had the highest carotenoid concentration (7.32%). Inoculation of plants with fungal treatments of G. rosea, S. constrictum and G. intraradices (separately) also significantly increased chlorophyll (a) concentration compared to control. Eventually, the results showed the beneficial effects of mycorrhizal symbiosis in T. polium plant that combined use of these fungi can have a much greater and better effect on improving plant growth conditions, as fungi exist in form of combination in nature.