Document Type : Research Paper

Authors

1 MSc Student Plant Breeding, Islamic Azad University Boroujerd, Iran

2 Assistant Professor , Research Institute of Forests and Range country, Iran

3 Professor, Research Institute of Forests and Range country, Chairman of the gene bank, Iran

Abstract

In order to study, the effect of pre-cooling treatment, osmotic potential and water stress on some population of two species of Satureja bachtiarica and Satureja sahendica was studied by a factorial experiment based on completely randomized design with three replications in laboratory and greenhouse conditions during one year in the gene bank of Institute of forests and range lands by the years of 2010-2011. In laboratory, treatment were including: cold and osmotic potential making of polyethylenglycol (PEG) 6000 Da in 5 concentrations (0, -0.3, -0.6, -0.9, -1.2 Mgapascal) compared with control. In greenhouse, treatment were including: cold and drought stress in five levels of field capacity (100,80,60,40,20) compared with control. Result showed that seed germination and seed emergence were decreased as rate of 33% and 6% in order of laboratory and greenhouse condition with increasing of osmotic potential and water stress while the ratio of root to shoot and ratio of dry weight to fresh weight was increased. In both experimental conditions, the most of the seed characteristics were higher with pre-cooling than other treatments. In response to osmotic potential in germinator, and dry stress in greenhouse, Bijar 2 population from Sahandy savory and piranshar from bachtiary savory showed more tolerant to dry stress due to have more vigor index and ratio of dry weight to fresh weight and they introduced as superior population.Comparing of the population showed that population of the takestan of Sahandy savory had lower tolerance.

Keywords

[1] Abdul - baki, A.A. and Anderson, J.D. (1975). Vigour determination in soybean seed by multiple criteria. Crop aci, 630-633.
[2] Alizadeh, M.A. and Isvand, H.R. (2004). Evaluation and the study of germination potential, speed of germination and vigour index of the seeds of two species of medicinal plant (Eruca sativa Lam.,Anthemis altissima L) under coold room anry storage condition. Iranian Journal of Medicinal and Aromatic plant Research, 20(3), 301-307.
[3] Allen, S.G., Dobrenz, A.K., Schonhorst, M.H. and Stoner, J.E. (1985). Heretability of Nacl tolerance in germination of alfalfa seed. Agron, J., 77, 99-101.
[4] Baba Khanlo, P., Mirza, M., Sefidkon, F., Ahmadi, L. and Asgari, F. (1998). Evaluation of the constituent composition of Essential oil in Ziziphora clinopodioides, Zataria mutaria multiflora, Z.tenuior. Iranian Journal of Medicinal and Aromat [19] Zic Plants, 2, 22-120.
[5] Chuanren, D., Bochu, W., Wanqian, L., Jin, C., Jie, L., Haan, z. (2004). Effect of chemical and physical factors to imporove the germination rate of Echinacea anqustifolia seed‌. Colloids and surfaces biointer faces, 37, 101-105.
[6] Emmerich, W.E. and Hardgree, S.P. (1990). Polyethylenglycol solution contact effection seed germination. Agron .J., 82, 1103-1107.
[7] Faker Baher, Z., Rezai, M.B., Mirza, M.M. and Abbaszadeh, B. (2001). Evaluation of Qualitive and Quantitive changing of Essential Oil of Satureja. Iranian Journal of Medicinal and Aromatic Plants,[p1]  11, 36-51.
[8] Goicochea, N., Antolin, M.C. and Sanchez, D.M. (1997). Gas exchange is related to hormone balancein mycorrhizal or nitrogen fixing alfalfa subjected to drought. Physiologia planturum, 100, 989-997.
[9] Hossienpour Ghazveni, A. (2010). Evaluation germination and vigour problem in four ecotype in laboratory and greenhouse Msc Thesis. Azad university –unit of Takestan, 1-102.
[10] Jamzad, Z. (2009). Thymus and Savory of Iran, Pub. Research Istitute of Forest and Rangeland, 81-132.
[11] Kochaky, A., Bromand, J. and Rezazadeh, Z. (2005). Evaluation of seed germination response to osmotic and matrix potential of polyethylenglycol and sodium coloride in different temperature, J. agronomical research of Iran, 3, 207-217.
[12] Lebaschi, M., Sharif Ashorabadi, H. and Mazaheri, D. (2003). Effect of dry stress on changing of hypersin. J. of Pagohesh and Sazandeghi, 58(1), 44-52.
[13] Lekh, R. and Khairwal, I.S. (1993). Evaluation of pearl millet hybrids and their parents for germ inability and field emergence. Indian Jour. Plant Physiol, 125-127.
[14] Lowlor, D.w. (1970). Absorption of polyethylenglycols by plants and Their effecte on plant growth, New phytol, 69.
[15] Maguire, J.D. (1962). Speed of germination: Aid in selection and evaluation for seedling vigour, Crop science, 2, 176-177.
[16] Michel, B.E. and Kaufman, M.R. (1973). The osmotic potential of polyethylenglycol 6000 plant physiology, 51.
[17] Omidbighy, R. (1997). The obtained strategy of production and technology of plant medicine No of Issue (1, 2 and 3). Publication of Ghods.
[18] Sarmadnia, Gh.H. (1996). Translation of seed technology. Published by Jahad university of Mashhad.
[19] Sedighi, M. and Porkermani, M. (1983). Un-uniform figure in arid zone. Translation- Publication of Ghods.
[20] Sefidkon, F., Jamzad, Z. and Barzandeh, M. (2004). Essential oil of Satureja bachtiarica as a full source of Karvakrol. Iranian Journal of Medicinal and Aromatic Plants, 20(4), 439-425.
[21] Waterman, p.g. and mole, s. (1994). Analysis of phenol and secondary metabolites. black well scientific publications. oxford, new york, 44-66.