Document Type : Research Paper


1 University of Zabol

2 Range and watershed management department, Water and Soil collage, University of Zabol

3 MSc student in Range management, Faculty of water and soil, University of Zabol


Germination is one of the most important stages of plant growth that may be affected by different stresses in ‎natural environments. This study was kind aimed to investigate the effects of substrate and different levels of ‎gibberellic and indole butyric acid on germination characteristics of Salvadora persica seeds in 2019. Two factors ‎was considered consist of substrate (in four types (1- pit moss, 2 - cocopit and 3 - pit moss 50% + sand 50% 4 - ‎Cocopit 50% + 50% sand), and gibberellic acid (in two levels 250 and 500 ppm) and indole butyric acid (in two ‎levels 250 and 500 ppm). Then, the effect of these two factors and distilled water as the control in three ‎replications on seed germination and seedling growth of Salvadora persica was investigated using a completely ‎randomized factorial design. The results showed that the substrate had a significant effect on germination ‎percentage, root length, shoot length, seedling, fresh root weight, shoot fresh weight and seed vigor index ‎‎(p < 0.01). Seed pretreatment with gibberellic and indole butyric acid hormones had a significant effect on all ‎studied characteristics. Interaction of substrate type and pretreatment with hormones had a significant effect on ‎germination percentage, root length, shoot length, seedling and seed vigor index (p < 0.01) and had no significant ‎effect on root and shoot fresh weight. The highest germination percentage was obtained in cocopeat and ‎gibberellic acid 250 ppm (73%).


[1]     Abdul, W., Mubaraka, P., Gelani, S. and S. Basrab. (2007). Pretreatment of seed with H2 O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins. Journal of Plant Physiology, 164, 283-294.
[2]     Ahmadloo, F., Tabari, T., Rahmani, A., Yousefzadeh, H. and Razagh Zadeh, M. (2009). Effect of ‎soil composition on seed germination of Pinus halepensis Mill. Iranian journal of Forest and ‎Poplar Research, 17(3), 403-394.‎
[3]     Ahmed, S.S., EL-Gengaihi, S.E.E., Ibrahim, M.E.S. and Schnug, E. (2008). Preliminary ohytochemical and propagation trial with Salvadora Persica L, Landbauforschung-VTI Agri culture and forestry Research, 58, 135-138.
[4]     Alaey, M., Naderi, R., Khalighi, A. and Salami, S.A. (2007). Effect of different factors on seed germination of Persian cyclamen (Cyclamen persicum mill.). Journal of Pajouhesh & Sazandegi, 67, 36-43.
[5]     Awang, Y., Shaharom, A.S., Mohammad, R.B. and Selamat, A. (2009). Chemical and physical characteristics of cocopeat-based media mixtures and their effects on the growth and development of Celosia cristata. American Journal of Agricultural and Biological Sciences, 4(1), 63-71.
[6]     Bahrani, A. and Pourreza, J. (2012). Gibberellic acid and salicylic acid effects on seed germination
and seedlings growth of wheat (Triticum aestivum L.) under salt stress condition. World
Applied Science Journal, 18(5), 633-641.
[7]     Blythe, G., Denlay, T. and Sibley, J.L. (2000). Influence of commercial auxin formulation on cutting of camellia cultivars. SNA R research conference, 45, 303-306.
[8]   Burun, B. and shahin, O. (2009).In vitro and in vivo germination of Cyclamen alpinum seeds. Tubitak. Turk Journal Botany, 33, 277-283.
[9]     Camberato, J. and Mccarty, B. (1999). Irrigation water quality: part I. Salinity. South CarolinaTurfgrass Foundation News, 6, 68.
[10] Davidson, H., Meckienburg, R. and Peterson, C. (1998). Nursery management: Administration and culture. Seconded prentice. Hall, Inc. New jersey.
[11] Delker, C., Zolman, B.K., Miersch, O. and Wasternack, C. (2007). Jasmonate biosynthesis in
Arabidopsis thaliana requires peroxisomal β-oxidation enzymes–Additional proof by properties
of pex6 and aim1. Phytochemistry, 68(12), 1642-1650.
[12] El-Araby, M.M. and Hegazi, A.Z. (2004). Responses of tomato seeds to hydro- and osomo- priming
and possible relations of some antioxidant enzyme and endogenous polyamine fractions.
Egyptian Journal of Biology, 6(1), 81-93.
[13] Ertan, E. and Alkan, G. (2018). Effects of different substrates on the germination and growth performance of chestnut nursery trees. Acta Hortic, 1220, 149-154.
[14] Esfandiari, A., Sadat Taghavi, T.,  Babalar, M. and  Delshad, M. (2008). The effect of using raw and NH 4-zeolite on yield and quality of tomato crop at reduced nitrogen concentration solution in hydroponic. Journal of Horticultural Sciences, 23 (2), 41-51.
[15] Farooq, M., Basra, S. M. A., Warraich, E. A. and Khaliq, A. (2006). Optimization of hydropriming techniques for rice seed invigoration. Seed Science. Technology, 34, 529-534.
[16] Ghassemi-Golezani, K., Asghar Aliloo, A., Valizadeh, M., and Moghaddam, M. (2008). Effects of hydro and osmo-priming on seed germination and field emergence of lentil (Lens culinaris Medik). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 36 (1), 29-33.
[17] Inden, H. and Torrres, A. (2004). Comparison of four substrates on the growth and quality of tomatoes. Acta Horticltur, 644, 205-210.
[18] Iqbal, G.M.A., Huda, S.M.S., Sujauddin, M. and Hossain, M.K. 2007. Effects of sludge on germination and initial growth performance of Leucaena leucocephala seedlings in the nursery. Journal of Forestry Research, 18 (3), 226-230.
[19] Isvand, H., Azarnia, M., Nazarian Firoozabadi, F. and Sharafi, R. (2012). Effects of Priming by Gibberellin and Abcsisic Acid on Emergence and some Physiological Characters of Chikpea (Cicer arietinum L.) Seedling under Dry and Irrigated Conditions. Iranian Journal of Field Crop Science, 42 (4), 789-797.
[20] Javanpour Heravi, R., Babalar M., Kashi A., Mirabdolbaqi M., and Asgari M.A. (2005). Effect of cirtain nutrient solution and growing media on qualitative and quantitative traits of tomato cv. Hamra in hydroponic system. Irainian Journal of Agriculture Science, 36 (4), 939-946.
[21] Javanshir, K. (1988). Vegetable of Bashagard area, Tehran University Press.
[22] Kaur, S., Gupta, A.K. and Kaur, N. (2000). Effect of G3, kinetin and indole acetic acid on Caohydrate metabolism in chickpea Seedlings germination under water stress. Plant Growth Regular, 30, 70-61.
[23] Kfuban Yerima, B., Mbakpor Ayuk, G., Kogge Enang, R., Guehjung, N. and Alphonse Tiamgne, Y. (2016). Germination and Early Seedling Growth of Moringa oleifera Lam with Different Seeds Soaking Time and Substrates at the Yongka Western Highlands Research Garden Park (YWHRGP) Nkwen-Bamenda, North-West Cameroon. American Journal of Plant Sciences, 7(15), 2173-2185.
[24] Khandan-Mirkohi, A., Moshrefi-Araghi, A.R., Haghdoost, L., Rashid- Rostami,F. and  Sahraii, S. (2015). The effect of rooting medium, cutting type and auxin (IBA) treatment on propagation of Arizona cypress (Cupressus arizonica var. glabra). Journal of Science and Technology of Greenhouse Culture, 5(4), 193-202.
[25] Khoshkhooy, M. (2003). Modern principles of horticulture, Shiraz University Press.
[26] Liptay, A. and Zariffa, N. (1993). Testing the morphological aspects of polyethylene glycol-primed tomato seeds with proportional odds analysis. Horticulture Science, 28(9), 881-883.
[27] Luiz piva, A., Junior Mezzalira, E., Santin, A., Sschwantes. D., Klein, J., Rampim, L., Villa, F., Yuji Tsutsumi, C. and Antonio Nava, G. (2013). Emergence and initial development of Cape -gooseberry (Physalis peruviana L.) seedlings with different substrate compositions. African Journal of Agricultural Research, 8(49), 6579-6584.
[28] Martinz, P.F. and Abad, M. (1992). Soilless culture of tomato in different mineral substrates. Acta Horticulture, 323, 251-259.
[29] Miransari, M. and Smith, D.L. (2014). Plant hormones and seed germination. Environmental and Experimental Botany, 99, 110-121.
[30] Mohammadi, L. and Shekari, F. (2015). Examination the effects of hydro-priming and priming by
salicylic acid on lentil aged seeds. International Journal of Agriculture and Crop Sciences, 8(3),
[31] Molahasani, H. Baghi, E. (2009). Substrates and their components in Soilless culture. First National Congress of Hydroponics and Production of greenhouse, 30/6/2009, Isfahan, Iran, pp. 332-334.
[32] Naba'ee, M., Roshandel, P. and Mohammad Khani, A. (2013). The effects of Plant Growth Regulators on Breaking Seed Dormancy in Silybum marianum L. Journal of Cell & Tissue, 4(1), 45-54.
[33] Noguera, P., Abad, M., Noguera, V., Puchades, R. and Maquieira, E. (2000). Coconut coir waste, a new and ecologically-friendly peat substitute. Acta Hort, 517, 279-286.
[34] Pandya, J, B., Gohil, R, H., Patolia, J, S., Shah, M, T. and Parmar, D, R. (2006). A study on Salicornia (S. brachiata Roxb) to salinity ingressed soils of India, International Journal of Agricultural Research, 1, 91-99.
[35] Power, P. and Fonteyn, P.J. (1995). Effect of oxygen concentration and substrate on seed germination and seedling growth of Texas wild rice (Zizania texana). Southwest. Nature, 40, 1-4.
[36] Ranjbar, R. and Taghavi, R. (2007). Possibility of using perlite, vermiculite and manure tobacco
Burley 21 in the floating method. The Tenth Congress of Soil Science, 26/8/2007, Karaj, Iran.
[37] Rashidi, S., Panahi, B., Hosseinifard, S. J. and Ebrahimi, F. (2015). The effect of vermicompost and animal manure on growth and flowering of marigold. Department of Horticulture, Islamic Azad University, Jiroft Branch.
[38] Rouhi, H.R., Aboutalebian, M.A., Moosavi, S.A., Karimi, F.A., Karimi, F., Saman, M. and Samadi, M. (2012). Change in several antioxidant enzymes activity of Berseem clover (Trifolium alexandrinum L.) by priming. International Journal of Agri Science, 2 (3), 237- 243.
[39] Saberi, M. and V. karimian. (2019). Influence of chemical stimulators to development, support and resistant of Datura Stramonium medicinal plant under stress allelopathic components of Eucalyptus camaldulensis. Rangeland, 12 (4), 401-410.
[40] Saberi, M., Tavili, A. and Miri, M. (2014).  Investigation the effects of different levels of gibberellic and salicylic acid on improvement of germination indices of Festuca arundinacea under stress with allelopathic compound. Journal of Natural Environment, 64 (4), 415-424
[41] Saberi, M. and A. Tavili. (2010). Evaluation different priming treatments influences on Puccinella distans germination characteristics. Iranian Journal of Range and Desert Research, 17 (1), 60-73.
[42] Saberi, M., Shahriari,A.R., Tarnian, F., Jafari, M. and Safari, H. (2011). Influence of Seed Priming on Germination and Seedling Range Species under Allelopathic Components. Frontiers of Agriculture in China, 2011, 5 (3), 310-321.
[43] Samiei, L., Khalighi, A., Kafi, M., Samavat, S. and Arghavani, M. (2005). An Investigation of Substitution of Peat Moss With Palm Tree Celluloid Wastes in Growing Aglaonema (Aglaonema Commutatum Cv. Silver Queen). Iranian Journal of Agriculture Science, 36 (2), 503-510.
[44] Seiadat, S. A., Moosavi, A. and Sharifzadeh, M. (2012). Effect of seed priming on antioxidant activity and germination characteristics of Maize seeds under different aging treatments. Research Journals of Seed Science, 5, 51-62.
[45] Shahinrokhsar, P., Shokrivahed, H., Asadi, M.E., Davari, K. and Peyvast, GH.A. (2010). Effect of Irrigation Managements and Substrates on some Quality and Quantity Parameters of Greenhose Tomato. Journal of Water and Soil Science, 14 (53), 53-63.
[46] Shahzad, M.A., Basra, M.F., Farooq, M., Rehman, H. and Saleem, B.A. (2007). Improving the germination and early seedling growth in melon (Cucumis melo L.) by pre-sowing salicylicate treatments. International Journal of Agricultural Biology,9, 550-554.
[47] Shokri, S., Zarei1, H., Alizadeh, M. (2014). Effect of rooting media on root production of semi-hardwood stem cuttings in weeping bottlebrush (Calistemon viminalis) under greenhouse conditions. Journal of Science and Technology of Greenhouse Culture, 5 (3), 173-183.
[48] Tavili, A., M. Saberi. and A. R. Shahriari. (2010). Effects of different treatments on improving seed germination and initial growth properties in Zygophyllum eurypterum Boiss. & Buhse and Zygophyllum eichwaldii C.A.M. Watershed Management Research Journal (Pajouhesh & Sazandegi), 86, 64-69.
[49] Taylor, A.G. (1997). Seed storage, germination and quality: 1-36. In: Wien. H. C. (Ed.) The Physiology of Vegetable Crops Wallingford, U.K: CAB International.
[50] Taylor, A.G. and Harman, G.E. (1990). Concepts and technologies of selected seed treatments.
Annuals Review Phytopathology, 28 (1), 321–339.
[51] Vamil, R., Aniat-ul-haq, R., Agnihotri, K. and Sharma, R. (2011). Effect of certain plant growth regulators on the seedling survival, biomass production and proline content of Bambusa arundinacea. Science Research Reporter, 1 (2), 44-48.
[52] Zhang, J., Siemann, E., Tian, B., Huang, W. and Ding, J. (2020). Differences in seed properties and germination between native and introduced populations of Triadica sebifera, Journal of Plant Ecology, 13(1), 70–77.