نشریه علمی - پژوهشی مرتع و آبخیزداری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار گروه مرتع‌داری، دانشکده منابع طبیعی، دانشگاه تربیت مدرس، ایران.

2 دانشجوی کارشناسی ارشد مرتع‌داری دانشکده منابع طبیعی، دانشگاه تربیت مدرس، ایران.

3 استادیار دانشگاه آزاد اسلامی واحد فیروزآباد، ایران.

4 استادیار مرکز تحقیقات کشاورزی و منابع طبیعی مازندران، ایران.

چکیده

در این تحقیق اثر استرس شوری روی تغییرات روزنه برگ‌های H. coronariumوH. criniferumبا استفاده از میکروسکوپ نوری بررسی شد. طرح به‌صورت آزمایش فاکتوریل شامل (تیمارهای گونه‌های گیاهی و سطح شوری صفر، 100، 200، 250 و 300 میلی‏مولار کلرید سدیم) بر پایه طرح کاملاً تصادفی در 4 تکرار و 50 عدد بذر در هر تکرار کشت گردید. بذرها در گلدان‌های پلاستیکی در ماسه‌های استریل شده رشد نمودند. همه گلدان‌ها با آب مقطر تا مرحله جوانه‌زنی آبیاری شدند، سپس با محلول هوگلند همراه با تیمارهای شوری به مدت 40 روز آبیاری ادامه یافت. درنهایت صفات روزنه اندازه‌گیری شدند. آنالیز آماری داده‌ها به‌وسیله نرم‌افزار SPSS انجام شد. اختلاف بین میانگین‌ها با استفاده از آزمون دانکن (05/0P <) مشخص گردید. نتایج نشان دادند کمترین مقدار طول روزنه، عرض روزنه و مساحت روزنه در تنش شوری 300 میلی مولار مشاهده شد. طول و عرض روزنه  با افزایش تنش شوری کاهش یافتند. طول روزنه 33/22 و 76/23 میکرومتر به ترتیب برای H. coronariumو. H. criniferumکاهش یافت، از آنجائی که عرض روزنه با 13/17 و 62/18 میکرومتر به ترتیب برای H. coronariumو.H. criniferumکاهش یافت. مساحت روزنه 11/397 و 83/442 میکرومترمربع  به ترتیب برای  H. coronariumوH. criniferumکاهش یافت. استرس شوری اثرات مثبت روی تراکم روزنه‌ها (میکرومتر مربع) داشتند. بیشترین تراکم روزنه‌ها در گونه H. criniferum در ترازهای 250 و 300 میلی مولار شوری به دست آمد. 

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Salinity Stress on Stomatal Variations of Hedysarum coronarium L. and Hedysarum criniferum Boiss.

نویسندگان [English]

  • Ghasem Ali Dianati Tilaki 1
  • Aliyeh Keshavarz 2
  • Bahram Amiri 3
  • Ehsan Sadati 4

1 Associate Professor of Tarbiat Modares University

2 Tarbiat Modares University

3 Islamic Azad University, Firoozabad Branch, Iran

4 Mazandaran, Research Center of Agricultural and Natural Resources

چکیده [English]

Effect of salt stress on the stomata variations was studied in Hedysarumcoronarium L. and HedysarumcriniferumBoiss., using optical microscope. The experimental design was a two factorial (species treatments and salt concentrations including 0, 100, 200, 250 mMNaCl) which arranged in a completely randomized design with four replicates and 50 seeds per replicate. Seeds were grown in plastic pots with sterile sand. All pots were irrigated by distilled water until germination stage. Then the pots were irrigated uniformly every other day with modified Hoagland’s nutrient solution during 40 days and finally stomata traits were measured. The data was analyzed using SPSS software. The difference between the means was compared using Duncan test (P<0.05). Results showed that the lowest amount of the stomata length, stomata width and area of stomata were observed at 300 Mm salinity stress. Length and width of stomata were reduced by increasing salinity stress. The length stomata decreased 22.33 and 23.76 (μm) for H. coronarium and H. criniferum respectively, while width stomata decreased by 17.13 and 18.62 (μm) respectively. The area of stomata decreased 397.11 and 442.83 (μm²) for H. coronarium and H. criniferum respectively. Salinity stress had positive effect on stomata density (mm²). The highest density of stomata was found in H. criniferumin 250 and 300 mM salinity levels.

کلیدواژه‌ها [English]

  • Salinity stress
  • stomata
  • Density
  • Hedysarum coronarium
  • Hedysarum criniferum
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